Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give controlled expression that is suitable for diverse biotechnology applications

L^2-Betti numbers of one-relator groups

We determine the L^2-Betti numbers of all one-relator groups and all surface-plus-one-relation groups (surface-plus-one-relation groups were introduced by Hempel who called them one-relator surface groups). In particular we show that for all such groups G, the L^2-Betti numbers b_n^{(2)}(G) are 0 for all n>1. We also obtain some information about the L^2-cohomology of left-orderable groups, and deduce the non-L^2 result that, in any left-orderable group of homological dimension one, all two-generator subgroups are free.Comment: 18 pages, version 3, minor changes. To appear in Math. An

Insight into nucleon structure from generalized parton distributions

The lowest three moments of generalized parton distributions are calculated in full QCD and provide new insight into the behavior of nucleon electromagnetic form factors, the origin of the nucleon spin, and the transverse structure of the nucleon.Comment: 3 pages, Lattice2003(Theoretical developments

Moments of nucleon spin-dependent generalized parton distributions

We present a lattice measurement of the first two moments of the spin-dependent GPD H-tilde(x,xi,t). From these we obtain the axial coupling constant and the second moment of the spin-dependent forward parton distribution. The measurements are done in full QCD using Wilson fermions. In addition, we also present results from a first exploratory study of full QCD using Asqtad sea and domain-wall valence fermions.Comment: Lattice2003(Theory), 3 pages, 3 figures, to appear in the Proceedings of Lattice 200

Hadronic physics with domain-wall valence and improved staggered sea quarks

With the advent of chiral fermion formulations, the simulation of light valence quarks has finally become realistic for numerical simulations of lattice QCD. The simulation of light dynamical quarks, however, remains one of the major challenges and is still an obstacle to realistic simulations. We attempt to meet this challenge using a hybrid combination of Asqtad sea quarks and domain-wall valence quarks. Initial results for the proton form factor and the nucleon axial coupling are presented.Comment: Two Talks presented at Lattice2004(spectrum), LaTex, 6 pages, 6 eps figure

Tobacco Coupons and Teenagers

To the Editor. Despite mounting scientific evidence suggesting a causal relationship between tobacco advertising and increased consumption of tobacco products, particularly among adolescents, promotion of tobacco products by tobacco manufacturers continues to increase

Brand Logo Recognition by Children Aged 3 to 6 Years: Mickey Mouse and Old Joe the Camel

Objective.—Little is known about the influence of advertising on very young children. We, therefore, measured product logo recognition by subjects aged 3 to 6 years. Design.—Children were instructed to match logos with one of 12 products pictured on a game board. Twenty-two logos were tested, including those representing children’s products, adult products, and those for two popular cigarette brands (Camel and Marlboro). Setting.—Preschools in Augusta and Atlanta, Ga. Participants.—A convenience sample of 229 children attending preschool. Results.—The children demonstrated high rates of logo recognition. When analyzed by product category, the level of recognition of cigarette logos was intermediate between children’s and adult products. The recognition rates of The Disney Channel logo and Old Joe (the cartoon character promoting Camel cigarettes) were highest in their respective product categories. Recognition rates increased with age. Approximately 30% of 3-year-old children correctly matched Old Joe with a picture of a cigarette compared with 91.3% of 6-year-old children. Conclusion.—Very young children see, understand, and remember advertising. Given the serious health consequences of smoking, the exposure of children to environmental tobacco advertising may represent an important health risk and should be studied further

Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosis

10.1007/s11373-005-9051-9Journal of Biomedical Science133433-44

Rapid Rule-out of Acute Myocardial Infarction With a Single High-Sensitivity Cardiac Troponin T Measurement Below the Limit of Detection: A Collaborative Meta-analysis.

Background: High-sensitivity assays for cardiac troponin T (hs-cTnT) are sometimes used to rapidly rule out acute myocardial infarction (AMI). Purpose: To estimate the ability of a single hs-cTnT concentration below the limit of detection (<0.005 µg/L) and a nonischemic electrocardiogram (ECG) to rule out AMI in adults presenting to the emergency department (ED) with chest pain. Data Sources: EMBASE and MEDLINE without language restrictions (1 January 2008 to 14 December 2016). Study Selection: Cohort studies involving adults presenting to the ED with possible acute coronary syndrome in whom an ECG and hs-cTnT measurements were obtained and AMI outcomes adjudicated during initial hospitalization. Data Extraction: Investigators of studies provided data on the number of low-risk patients (no new ischemia on ECG and hs-cTnT measurements <0.005 µg/L) and the number who had AMI during hospitalization (primary outcome) or a major adverse cardiac event (MACE) or death within 30 days (secondary outcomes), by risk classification (low or not low risk). Two independent epidemiologists rated risk of bias of studies. Data Synthesis: Of 9241 patients in 11 cohort studies, 2825 (30.6%) were classified as low risk. Fourteen (0.5%) low-risk patients had AMI. Sensitivity of the risk classification for AMI ranged from 87.5% to 100% in individual studies. Pooled estimated sensitivity was 98.7% (95% CI, 96.6% to 99.5%). Sensitivity for 30-day MACEs ranged from 87.9% to 100%; pooled sensitivity was 98.0% (CI, 94.7% to 99.3%). No low-risk patients died. Limitation: Few studies, variation in timing and methods of reference standard troponin tests, and heterogeneity of risk and prevalence of AMI across studies. Conclusion: A single hs-cTnT concentration below the limit of detection in combination with a nonischemic ECG may successfully rule out AMI in patients presenting to EDs with possible emergency acute coronary syndrome. Primary Funding Source: Emergency Care Foundation